ATCC Medium: 18 Tryptic Soy Agar/Broth (Soybean-Casein Digest Medium, USP)
Agar Medium
Tryptic Soy Agar (BD 236950)…………………..40 g
DI Water……………………………………………1000 ml
Autoclave at 121? C.
Broth Medium
Tryptic Soy Broth (BD cat 211825)…………….30 g
DI Water…………………………………………..1000 ml
Autoclave at 121? C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Tryptic Soy Agar Composition
Tryptone………………………………….………17 g
Soytone…………………………………………..3 g
Dextrose………………………………….………2.5 g
NaCl………………………………………..……..5.0 g
K2HPO4……………………………………..…...2.5 g
Agar………………………………………..……..15 g
Final pH 7.3 +/- 0.2.
*Omit agar for broth medium. It should be noted that this formulation contains dextrose.
The commercially available Tryptic Soy Agars do not contain dextrose.