ATCC medium: 1658 MMS Medium for Thermotoga neapolitana
NaCl..................................... . 6.93 g
7H2O . MgSO4 .............................1.75 g
MgCl2 . 6H2O .............................1.38 g
KCl...................................... 0.16 g
NaBr.................................... 25.0 mg
H3BO3 .................................... 7.5 mg
SrCl2 . 6H2O ............................. 3.8 mg
KI....................................... 0.025 mg
CaCl2 .................................... 0.38 g
KH2PO4 ................................... 0.5 g
Na2S . 9H2O .............................. 0.5 g
(NH4)2Ni(SO4)2 ............................ 2.0 mg
Wolfe's Mineral Solution (see below).... 15.0 ml
Resazurin................................ 1.0 mg
Starch................................... 5.0 g
Distilled water to....................... 1.0 L
Adjust final pH to 6.5 with H2SO4. Prepare anaerobically under 80% N2, 20% CO2. Autoclave.
Wolfe's Mineral Solution: Available from ATCC as a sterile ready-to-use liquid (Trace Mineral Supplement, catalog no.MD-TMS.)
Nitrilotriacetic acid.................... 1.5 g
MgSO4 . 7H2O ............................. 3.0 g
MnSO4 . H2O .............................. 0.5 g
NaCl..................................... 1.0 g
FeSO4 . 7H2O ............................. 0.1 g
CoCl2 . 6H2O ............................. 0.1 g
CaCl2 .................................... 0.1 g
ZnSO4 . 7H2O ............................. 0.1 g
CuSO4 . 5H2O ............................. 0.01 g
AlK(SO4)2 . 12H2O......................... 0.01 g
H3BO3 .................................... 0.01 g
Na2MoO4 . 2H2O............................ 0.01 g
Distilled water.......................... 1.0 L
Add nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.