ATCC medium: 1029 LIT medium
Liver Infusion Broth (BD 226920)......... 9.0 g
Tryptose (BD 211713)..................... 5.0 g
NaCl..................................... 1.0 g
Na2HPO4 .................................. 8.0 g
KCl...................................... 0.4 g
Glucose.................................. 1.0 g
Fetal bovine serum (heat-inactivated).. 100.0 ml
Hemin................................... 10.0 mg
Distilled water to....................... 1.0 L
Adjust pH to 7.2 and filter-sterilize. Dispense aseptically in 5.0 ml aliquots into 16 X 125 screw-capped test tubes.