ATCC medium: 706 M medium
Infuse 5.0 g of Beef (BD 213110) in 200.0 ml of distilled water by bringing to a rapid boil for 3-5 minutes, stirring constantly.
Filter through Whatman #2V filter paper then add:
Neopeptone, DIFCO (BD 211681)...... 4.0 g
NaCl............................... 1.6 g
KCl................................ 0.04 g
CaCl2 .............................. 0.06 g
Glucose............................ 0.5 g
KH2PO4 ............................. 0.06 g
Heat to boiling and restore volume to 200.0 ml. Adjust pH to 7.2 with 1 N NaOH. Filter through Whatman #2V filter paper. Autoclave for 20 minutes at 121C. Cool and aseptically add lysed rabbit blood solution (1:1 whole rabbit blood to sterile distilled water, frozen and thawed twice) to a final concentration of 20%.